LEC- talked about chapter 5,8,37
LAB- done with unknowns
Friday, August 3, 2012
Wednesday, August 1, 2012
Monday, July 30, 2012
unknown data chart
Unknown Data Chart
Test Date
|
Test Performed
|
Date Test Interpreted
|
Test Results
|
07-24-12
|
Made a TSA isolation plate at 37°C
|
07-25-12
|
Two different isolated colonies
1. Small
white colonies
2. Larger
tan colonies
|
07-24-12
|
Made a TSA isolation plate at 30°C
|
07-25-12
|
Two different isolated colonies
1. Small
white colonies
2. Larger
tan colonies
|
07-24-12
|
Made a PEA isolation plate at 37°C
|
07-25-12
|
Small white colonies grew
|
07-24-12
|
Made a Mac isolation plate at 37°C
|
07-25-12
|
Larger tan colonies grew
|
07-24-12
|
Made 2 gram stain slides with the same results
|
07-24-12
|
Positive: purple cocci
Negative: pink/red bacilli (rods)
|
07-25-12
|
Made a slant from small white isolated colony (30°C)
|
07-26-12
|
Isolated growth
(gram positive)
|
07-25-12
|
Made slant from larger tan isolated colony (30°C)
|
07-26-12
|
Isolated growth
(gram negative)
|
07-25-12
|
Made a gram stain from small white isolated colony (30°C)
|
07-25-12
|
Positive: purple cocci
(isolated)
|
07-25-12
|
Made a gram stain from larger tan isolated colony (30°C)
|
07-25-12
|
Negative: pink/red bacilli (rods)
(isolated)
|
07-26-12
|
Made new negative slant
|
07-27-12
|
Isolated growth
|
07-26-12
|
Made new positive slant
|
07-27-12
|
Isolated growth
|
07-26-12
|
Glucose test with gram negative slant
|
07-27-12
|
Ferments in aerobic and anaerobic conditions
|
07-26-12
|
Catalase test with gram positive slant
|
07-26-12
|
Negative reaction there were no bubbles
|
07-26-12
|
Checked previous PEA results for hemolysis test
|
07-26-12
|
Alpha (partial) hemolysis
|
07-26-12
|
Bile esculin test with gram positive slant
|
07-27-12
|
Positive resulted turned black
|
07-27-12
|
Made new gram negative slant
|
07-28-12
|
Isolated growth
|
07-27-12
|
Made new gram positive slant
|
07-28-12
|
Isolated growth
|
07-27-12
|
Glycerol PR broth test with gram positive
|
07-28-12
|
(after 24 hours) Orange and no bubbles (-/-)
(after 120 hours) Yellow/ferments and no bubbles (A/-) *Enterococcus faecalis* |
07-27-12
|
TSI test with gram negative to check results
|
07-28-12
|
fermented on bottom, and defiantly a pungent smell
|
07-27-12
|
Oxidase test with gram negative
|
07-27-12
|
Turned purple almost instantly *Aeromonas Hydrophila*
|
07-27-12
|
Motility test with gram negative to check results
|
07-28-12
|
purple where it was poked in and a slight purple coloration around
the entire tube
|
07-28-12
|
Made new gram negative slant
|
07-30-12
|
Isolated growth
|
07-28-12
|
Made new gram positive slant
|
07-30-12
|
Isolated growth
|
07-28-12
|
Mannitol broth test to check gram positive results
|
07-30-12
|
Positive growth, fermented, turned yellow but no gas was produced
|
TSA 37°C
TSA 30°C
Mac
PEA
Gram Positive: purple cocci
Gram negative: red/pink rods
Isolated gromth
Catalase: negative
Glucose: ferment
Bile esculin: positive
Oxidase: positive
TSI: ferments
Motility: positive
Glyceral PR broth: (after 24 hours)
Glyceral PR broth: (after 120 hours): positive, ferments
Mannitol PR broth: positive, no bubbles
All my experiments
7-30-12
LEC- review game
LAB- worked on unknowns
LAB- worked on unknowns
Test Date
|
Test Performed
|
Date Test Interpreted
|
Test Results
|
07-24-12
|
Made a TSA isolation plate at 37°C
|
07-25-12
|
Two different isolated colonies
1. Small
white colonies
2. Larger
tan colonies
|
07-24-12
|
Made a TSA isolation plate at 30°C
|
07-25-12
|
Two different isolated colonies
1. Small
white colonies
2. Larger
tan colonies
|
07-24-12
|
Made a PEA isolation plate at 37°C
|
07-25-12
|
Small white colonies grew
|
07-24-12
|
Made a Mac isolation plate at 37°C
|
07-25-12
|
Larger tan colonies grew
|
07-24-12
|
Made 2 gram stain slides with the same results
|
07-24-12
|
Positive: purple cocci
Negative: pink/red bacilli (rods)
|
07-25-12
|
Made a slant from small white isolated colony (30°C)
|
07-26-12
|
Isolated growth
(gram positive)
|
07-25-12
|
Made slant from larger tan isolated colony (30°C)
|
07-26-12
|
Isolated growth
(gram negative)
|
07-25-12
|
Made a gram stain from small white isolated colony (30°C)
|
07-25-12
|
Positive: purple cocci
(isolated)
|
07-25-12
|
Made a gram stain from larger tan isolated colony (30°C)
|
07-25-12
|
Negative: pink/red bacilli (rods)
(isolated)
|
07-26-12
|
Made new negative slant
|
07-27-12
|
Isolated growth
|
07-26-12
|
Made new positive slant
|
07-27-12
|
Isolated growth
|
07-26-12
|
Glucose test with gram negative slant
|
07-27-12
|
Ferments in aerobic and anaerobic conditions
|
07-26-12
|
Catalase test with gram positive slant
|
07-26-12
|
Negative reaction there were no bubbles
|
07-26-12
|
Checked previous PEA results for hemolysis test
|
07-26-12
|
Alpha (partial) hemolysis
|
07-26-12
|
Bile esculin test with gram positive slant
|
07-27-12
|
Positive resulted turned black
|
07-27-12
|
Made new gram negative slant
|
07-28-12
|
Isolated growth
|
07-27-12
|
Made new gram positive slant
|
07-28-12
|
Isolated growth
|
07-27-12
|
Glycerol PR broth test with gram positive
|
07-228-12
|
Orange and no bubbles (-/-) *Enterococcus saccharolyticus*
|
07-27-12
|
TSI test with gram negative to check results
|
07-28-12
|
fermented on bottom, and defiantly a pungent smell
|
07-27-12
|
Oxidase test with gram negative
|
07-27-12
|
Turned purple almost instantly *Aeromonas Hydrophila*
|
07-27-12
|
Motility test with gram negative to check results
|
07-28-12
|
purple where it was poked in and a slight purple coloration around
the entire tube
|
07-28-12
|
Made new gram negative slant
|
07-30-12
|
Isolated growth
|
07-28-12
|
Made new gram positive slant
|
07-30-12
|
Isolated growth
|
07-28-12
|
Mannitol broth test to check gram positive results
|
07-30-12
|
Positive growth, fermented, turned yellow but no gas was produced
|
Mannitol PR broth
Sunday, July 29, 2012
7-28-12
*came in on Saturday to work on unknowns*
TSI (after 24 hours)
Test Date
|
Test Performed
|
Date Test Interpreted
|
Test Results
|
07-24-12
|
Made a TSA isolation plate at 37°C
|
07-25-12
|
Two different isolated colonies
1. Small
white colonies
2. Larger
tan colonies
|
07-24-12
|
Made a TSA isolation plate at 30°C
|
07-25-12
|
Two different isolated colonies
1. Small
white colonies
2. Larger
tan colonies
|
07-24-12
|
Made a PEA isolation plate at 37°C
|
07-25-12
|
Small white colonies grew
|
07-24-12
|
Made a Mac isolation plate at 37°C
|
07-25-12
|
Larger tan colonies grew
|
07-24-12
|
Made 2 gram stain slides with the same results
|
07-24-12
|
Positive: purple cocci
Negative: pink/red bacilli (rods)
|
07-25-12
|
Made a slant from small white isolated colony (30°C)
|
07-26-12
|
Isolated growth
(gram positive)
|
07-25-12
|
Made slant from larger tan isolated colony (30°C)
|
07-26-12
|
Isolated growth
(gram negative)
|
07-25-12
|
Made a gram stain from small white isolated colony (30°C)
|
07-25-12
|
Positive: purple cocci
(isolated)
|
07-25-12
|
Made a gram stain from larger tan isolated colony (30°C)
|
07-25-12
|
Negative: pink/red bacilli (rods)
(isolated)
|
07-26-12
|
Made new negative slant
|
07-27-12
|
Isolated growth
|
07-26-12
|
Made new positive slant
|
07-27-12
|
Isolated growth
|
07-26-12
|
Glucose test with gram negative slant
|
07-27-12
|
Ferments in aerobic and anaerobic conditions
|
07-26-12
|
Catalase test with gram positive slant
|
07-26-12
|
Negative reaction there were no bubbles
|
07-26-12
|
Checked previous PEA results for hemolysis test
|
07-26-12
|
Alpha (partial) hemolysis
|
07-26-12
|
Bile esculin test with gram positive slant
|
07-27-12
|
Positive resulted turned black
|
07-27-12
|
Made new gram negative slant
|
07-28-12
|
Isolated growth
|
07-27-12
|
Made new gram positive slant
|
07-28-12
|
Isolated growth
|
07-27-12
|
Glycerol PR broth test with gram positive
|
07-228-12
|
Orange and no bubbles (-/-) *Enterococcus saccharolyticus* (still
checking)
|
07-27-12
|
TSI test with gram negative to check results
|
07-28-12
|
(after 24 hours) fermented on bottom
(after 72 hours)
|
07-27-12
|
Oxidase test with gram negative
|
07-27-12
|
Turned purple almost instantly *Aeromonas Hydrophila* (still
checking)
|
07-27-12
|
Motility test with gram negative to check results
|
07-28-12
|
(after 24 hours) purple where it was poked in and a slight change in
purple coloration around the entire tube
(after 72 hours)
|
07-28-12
|
Made new gram negative slant
|
07-30-12
|
|
07-28-12
|
Made new gram positive slant
|
07-30-12
|
|
07-28-12
|
Mannitol broth test to check gram positive results
|
07-30-12
|
|
motility (after 24 hours)
glycerol PR broth
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