7-30-12

LEC- review game
LAB- worked on unknowns

Test Date	Test Performed	Date Test Interpreted	Test Results
07-24-12	Made a TSA isolation plate at 37°C	07-25-12	Two different isolated colonies 1.       Small white colonies 2.       Larger tan colonies
07-24-12	Made a TSA isolation plate at 30°C	07-25-12	Two different isolated colonies 1.       Small white colonies 2.       Larger tan colonies
07-24-12	Made a PEA isolation plate at 37°C	07-25-12	Small white colonies grew
07-24-12	Made a Mac isolation plate at 37°C	07-25-12	Larger tan colonies grew
07-24-12	Made 2 gram stain slides with the same results	07-24-12	Positive: purple cocci Negative: pink/red bacilli (rods)
07-25-12	Made a slant from small white isolated colony (30°C)	07-26-12	Isolated growth (gram positive)
07-25-12	Made slant from larger tan isolated colony (30°C)	07-26-12	Isolated growth (gram negative)
07-25-12	Made a gram stain from small white isolated colony (30°C)	07-25-12	Positive: purple cocci (isolated)
07-25-12	Made a gram stain from larger tan isolated colony (30°C)	07-25-12	Negative: pink/red bacilli (rods) (isolated)
07-26-12	Made new negative slant	07-27-12	Isolated growth
07-26-12	Made new positive slant	07-27-12	Isolated growth
07-26-12	Glucose test with gram negative slant	07-27-12	Ferments in aerobic and anaerobic conditions
07-26-12	Catalase test with gram positive slant	07-26-12	Negative reaction there were no bubbles
07-26-12	Checked previous PEA results for hemolysis test	07-26-12	Alpha (partial) hemolysis
07-26-12	Bile esculin test with gram positive slant	07-27-12	Positive resulted turned black
07-27-12	Made new gram negative slant	07-28-12	Isolated growth
07-27-12	Made new gram positive slant	07-28-12	Isolated growth
07-27-12	Glycerol PR broth test with gram positive	07-228-12	Orange and no bubbles (-/-) *Enterococcus saccharolyticus*
07-27-12	TSI test with gram negative to check results	07-28-12	fermented on bottom, and defiantly a pungent smell
07-27-12	Oxidase test with gram negative	07-27-12	Turned purple almost instantly *Aeromonas Hydrophila*
07-27-12	Motility test with gram negative to check results	07-28-12	purple where it was poked in and a slight purple coloration around the entire tube
07-28-12	Made new gram negative slant	07-30-12	Isolated growth
07-28-12	Made new gram positive slant	07-30-12	Isolated growth
07-28-12	Mannitol broth test to check gram positive results	07-30-12	Positive growth, fermented, turned yellow but no gas was produced

Mannitol PR broth