Sunday, July 29, 2012

7-27-12

LEC- talked about diseases
LAB- practical #3 and unknown experiments


Test Date
Test Performed
Date Test Interpreted
Test Results
07-24-12
Made a TSA isolation plate at 37°C
07-25-12
Two different isolated colonies
1.       Small white colonies
2.       Larger tan colonies
07-24-12
Made a TSA isolation plate at 30°C
07-25-12
Two different isolated colonies
1.       Small white colonies
2.       Larger tan colonies
07-24-12
Made a PEA isolation plate at 37°C
07-25-12
Small white colonies grew
07-24-12
Made a Mac isolation plate at 37°C
07-25-12
Larger tan colonies grew
07-24-12
Made 2 gram stain slides with the same results
07-24-12
Positive: purple cocci
Negative: pink/red bacilli (rods)
07-25-12
Made a slant from small white isolated colony (30°C)
07-26-12
Isolated growth
(gram positive)
07-25-12
Made slant from larger tan isolated colony (30°C)
07-26-12
Isolated growth
(gram negative)
07-25-12
Made a gram stain from small white isolated colony (30°C)
07-25-12
Positive: purple cocci
(isolated)
07-25-12
Made a gram stain from larger tan isolated colony (30°C)
07-25-12
Negative: pink/red bacilli (rods)
(isolated)
07-26-12
Made new negative slant
07-27-12
Isolated growth
07-26-12
Made new positive slant
07-27-12
Isolated growth
07-26-12
Glucose test with gram negative slant
07-27-12
Ferments in aerobic and anaerobic conditions
07-26-12
Catalase test with gram positive slant
07-26-12
Negative reaction there were no bubbles
07-26-12
Checked previous PEA results for hemolysis test
07-26-12
Alpha (partial) hemolysis
07-26-12
Bile esculin test with gram positive slant
07-27-12
 Positive resulted turned black
07-27-12
Made new gram negative slant
07-28-12
Isolated growth
07-27-12
Made new gram positive slant
07-28-12
Isolated growth
07-27-12
Glycerol PR broth test with gram positive
07-228-12
Orange and no bubbles (-/-) *Enterococcus saccharolyticus* (still checking)
07-27-12
TSI test with gram negative to check results
07-28-12
(after 24 hours) fermented on bottom
(after 72 hours)
07-27-12
Oxidase test with gram negative
07-27-12
Turned purple almost instantly *Aeromonas Hydrophila* (still checking)
07-27-12
Motility test with gram negative to check results

07-28-12
(after 24 hours) purple where it was poked in and a slight change in purple coloration around the entire tube
(after 72 hours)






glucose test

bile esculin test

oxidase test

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